Microscopy:Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
Article Snippet: .. LaserMask sand carving film (Rayzist Photomask, Inc.) Microcentrifuge Micro-spin column (Bio-Rad #732-6204) MicroSpin S-400 HR Columns (GE Healthcare #27-5140-01) Nikon Eclipse TE2000-U microscope with TIRF attachment; equipped as described in Equipment Setup PEEK tubing (Upchurch Scientific #1532, 1.59 mm outer diameter, 0.5 mm inner diameter) QIAquick PCR purification kit (Qiagen #28104) Silicon carbide, 220 grit (Electro Abrasives #220-SIC) HARMFUL inhalation hazard; wear mask Silicone tubing, 0.8 mm inner diameter, 0.8 mm wall thickness (Bio-Rad #731-8210) Syringe pump (KD Scientific model KDS220) Thermocycler for PCR Three-way flow, four-port switching valve (Upchurch Scientific V-100T) Ultraviolet curing optical adhesive (Norland Optical Adhesive #74 #7404) Vacuum chamber connected to a vacuum source for degassing solutions (Thermo Scientific #5305-0609, house vacuum supply @ 350 mm Hg) ..
Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
Article Snippet: Alternative UV sources capable of delivering 4.5 Joules/cm 2 between 320 – 400 nm may be used. (HARMFUL operate with UV-protection goggles) iQ imaging software (Andor) Laser Engraver: 30 watt Mini 24 Laser System (Epilog Laser) controlled by the computer running Corel Draw or Adobe Illustrator. .. LaserMask sand carving film (Rayzist Photomask, Inc.) Microcentrifuge Micro-spin column (Bio-Rad #732-6204) MicroSpin S-400 HR Columns (GE Healthcare #27-5140-01) Nikon Eclipse TE2000-U microscope with TIRF attachment; equipped as described in Equipment Setup PEEK tubing (Upchurch Scientific #1532, 1.59 mm outer diameter, 0.5 mm inner diameter) QIAquick PCR purification kit (Qiagen #28104) Silicon carbide, 220 grit (Electro Abrasives #220-SIC) HARMFUL inhalation hazard; wear mask Silicone tubing, 0.8 mm inner diameter, 0.8 mm wall thickness (Bio-Rad #731-8210) Syringe pump (KD Scientific model KDS220) Thermocycler for PCR Three-way flow, four-port switching valve (Upchurch Scientific V-100T) Ultraviolet curing optical adhesive (Norland Optical Adhesive #74 #7404) Vacuum chamber connected to a vacuum source for degassing solutions (Thermo Scientific #5305-0609, house vacuum supply @ 350 mm Hg) Equipment Setup Microscope Many published protocols exist for the construction of a suitable optical trapping microscope (for examples see Amitani et al . and Lee et al . 13 , 22 ). .. The instrument we constructed in the present protocol was developed from a Nikon Eclipse TE2000-U platform with a TIRF attachment (Nikon), equipped with a CFI Plan Apo TIRF 100×, 1.45 N.A., oil-immersed objective; 13 a schematic view of this setup is shown in .
Polymerase Chain Reaction:Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
Article Snippet: .. LaserMask sand carving film (Rayzist Photomask, Inc.) Microcentrifuge Micro-spin column (Bio-Rad #732-6204) MicroSpin S-400 HR Columns (GE Healthcare #27-5140-01) Nikon Eclipse TE2000-U microscope with TIRF attachment; equipped as described in Equipment Setup PEEK tubing (Upchurch Scientific #1532, 1.59 mm outer diameter, 0.5 mm inner diameter) QIAquick PCR purification kit (Qiagen #28104) Silicon carbide, 220 grit (Electro Abrasives #220-SIC) HARMFUL inhalation hazard; wear mask Silicone tubing, 0.8 mm inner diameter, 0.8 mm wall thickness (Bio-Rad #731-8210) Syringe pump (KD Scientific model KDS220) Thermocycler for PCR Three-way flow, four-port switching valve (Upchurch Scientific V-100T) Ultraviolet curing optical adhesive (Norland Optical Adhesive #74 #7404) Vacuum chamber connected to a vacuum source for degassing solutions (Thermo Scientific #5305-0609, house vacuum supply @ 350 mm Hg) ..
Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
Article Snippet: Alternative UV sources capable of delivering 4.5 Joules/cm 2 between 320 – 400 nm may be used. (HARMFUL operate with UV-protection goggles) iQ imaging software (Andor) Laser Engraver: 30 watt Mini 24 Laser System (Epilog Laser) controlled by the computer running Corel Draw or Adobe Illustrator. .. LaserMask sand carving film (Rayzist Photomask, Inc.) Microcentrifuge Micro-spin column (Bio-Rad #732-6204) MicroSpin S-400 HR Columns (GE Healthcare #27-5140-01) Nikon Eclipse TE2000-U microscope with TIRF attachment; equipped as described in Equipment Setup PEEK tubing (Upchurch Scientific #1532, 1.59 mm outer diameter, 0.5 mm inner diameter) QIAquick PCR purification kit (Qiagen #28104) Silicon carbide, 220 grit (Electro Abrasives #220-SIC) HARMFUL inhalation hazard; wear mask Silicone tubing, 0.8 mm inner diameter, 0.8 mm wall thickness (Bio-Rad #731-8210) Syringe pump (KD Scientific model KDS220) Thermocycler for PCR Three-way flow, four-port switching valve (Upchurch Scientific V-100T) Ultraviolet curing optical adhesive (Norland Optical Adhesive #74 #7404) Vacuum chamber connected to a vacuum source for degassing solutions (Thermo Scientific #5305-0609, house vacuum supply @ 350 mm Hg) Equipment Setup Microscope Many published protocols exist for the construction of a suitable optical trapping microscope (for examples see Amitani et al . and Lee et al . 13 , 22 ). .. The instrument we constructed in the present protocol was developed from a Nikon Eclipse TE2000-U platform with a TIRF attachment (Nikon), equipped with a CFI Plan Apo TIRF 100×, 1.45 N.A., oil-immersed objective; 13 a schematic view of this setup is shown in .
Purification:Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
Article Snippet: .. LaserMask sand carving film (Rayzist Photomask, Inc.) Microcentrifuge Micro-spin column (Bio-Rad #732-6204) MicroSpin S-400 HR Columns (GE Healthcare #27-5140-01) Nikon Eclipse TE2000-U microscope with TIRF attachment; equipped as described in Equipment Setup PEEK tubing (Upchurch Scientific #1532, 1.59 mm outer diameter, 0.5 mm inner diameter) QIAquick PCR purification kit (Qiagen #28104) Silicon carbide, 220 grit (Electro Abrasives #220-SIC) HARMFUL inhalation hazard; wear mask Silicone tubing, 0.8 mm inner diameter, 0.8 mm wall thickness (Bio-Rad #731-8210) Syringe pump (KD Scientific model KDS220) Thermocycler for PCR Three-way flow, four-port switching valve (Upchurch Scientific V-100T) Ultraviolet curing optical adhesive (Norland Optical Adhesive #74 #7404) Vacuum chamber connected to a vacuum source for degassing solutions (Thermo Scientific #5305-0609, house vacuum supply @ 350 mm Hg) ..
Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
Article Snippet: Alternative UV sources capable of delivering 4.5 Joules/cm 2 between 320 – 400 nm may be used. (HARMFUL operate with UV-protection goggles) iQ imaging software (Andor) Laser Engraver: 30 watt Mini 24 Laser System (Epilog Laser) controlled by the computer running Corel Draw or Adobe Illustrator. .. LaserMask sand carving film (Rayzist Photomask, Inc.) Microcentrifuge Micro-spin column (Bio-Rad #732-6204) MicroSpin S-400 HR Columns (GE Healthcare #27-5140-01) Nikon Eclipse TE2000-U microscope with TIRF attachment; equipped as described in Equipment Setup PEEK tubing (Upchurch Scientific #1532, 1.59 mm outer diameter, 0.5 mm inner diameter) QIAquick PCR purification kit (Qiagen #28104) Silicon carbide, 220 grit (Electro Abrasives #220-SIC) HARMFUL inhalation hazard; wear mask Silicone tubing, 0.8 mm inner diameter, 0.8 mm wall thickness (Bio-Rad #731-8210) Syringe pump (KD Scientific model KDS220) Thermocycler for PCR Three-way flow, four-port switching valve (Upchurch Scientific V-100T) Ultraviolet curing optical adhesive (Norland Optical Adhesive #74 #7404) Vacuum chamber connected to a vacuum source for degassing solutions (Thermo Scientific #5305-0609, house vacuum supply @ 350 mm Hg) Equipment Setup Microscope Many published protocols exist for the construction of a suitable optical trapping microscope (for examples see Amitani et al . and Lee et al . 13 , 22 ). .. The instrument we constructed in the present protocol was developed from a Nikon Eclipse TE2000-U platform with a TIRF attachment (Nikon), equipped with a CFI Plan Apo TIRF 100×, 1.45 N.A., oil-immersed objective; 13 a schematic view of this setup is shown in .
Adhesive:Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
Article Snippet: .. LaserMask sand carving film (Rayzist Photomask, Inc.) Microcentrifuge Micro-spin column (Bio-Rad #732-6204) MicroSpin S-400 HR Columns (GE Healthcare #27-5140-01) Nikon Eclipse TE2000-U microscope with TIRF attachment; equipped as described in Equipment Setup PEEK tubing (Upchurch Scientific #1532, 1.59 mm outer diameter, 0.5 mm inner diameter) QIAquick PCR purification kit (Qiagen #28104) Silicon carbide, 220 grit (Electro Abrasives #220-SIC) HARMFUL inhalation hazard; wear mask Silicone tubing, 0.8 mm inner diameter, 0.8 mm wall thickness (Bio-Rad #731-8210) Syringe pump (KD Scientific model KDS220) Thermocycler for PCR Three-way flow, four-port switching valve (Upchurch Scientific V-100T) Ultraviolet curing optical adhesive (Norland Optical Adhesive #74 #7404) Vacuum chamber connected to a vacuum source for degassing solutions (Thermo Scientific #5305-0609, house vacuum supply @ 350 mm Hg) ..
Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
Article Snippet: Alternative UV sources capable of delivering 4.5 Joules/cm 2 between 320 – 400 nm may be used. (HARMFUL operate with UV-protection goggles) iQ imaging software (Andor) Laser Engraver: 30 watt Mini 24 Laser System (Epilog Laser) controlled by the computer running Corel Draw or Adobe Illustrator. .. LaserMask sand carving film (Rayzist Photomask, Inc.) Microcentrifuge Micro-spin column (Bio-Rad #732-6204) MicroSpin S-400 HR Columns (GE Healthcare #27-5140-01) Nikon Eclipse TE2000-U microscope with TIRF attachment; equipped as described in Equipment Setup PEEK tubing (Upchurch Scientific #1532, 1.59 mm outer diameter, 0.5 mm inner diameter) QIAquick PCR purification kit (Qiagen #28104) Silicon carbide, 220 grit (Electro Abrasives #220-SIC) HARMFUL inhalation hazard; wear mask Silicone tubing, 0.8 mm inner diameter, 0.8 mm wall thickness (Bio-Rad #731-8210) Syringe pump (KD Scientific model KDS220) Thermocycler for PCR Three-way flow, four-port switching valve (Upchurch Scientific V-100T) Ultraviolet curing optical adhesive (Norland Optical Adhesive #74 #7404) Vacuum chamber connected to a vacuum source for degassing solutions (Thermo Scientific #5305-0609, house vacuum supply @ 350 mm Hg) Equipment Setup Microscope Many published protocols exist for the construction of a suitable optical trapping microscope (for examples see Amitani et al . and Lee et al . 13 , 22 ). .. The instrument we constructed in the present protocol was developed from a Nikon Eclipse TE2000-U platform with a TIRF attachment (Nikon), equipped with a CFI Plan Apo TIRF 100×, 1.45 N.A., oil-immersed objective; 13 a schematic view of this setup is shown in .
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